Large-scale cultivation of adventitious roots of Echinacea purpurea in airlift bioreactors for the production of chichoric acid, chlorogenic acid and caftaric acid |
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Authors: | Chun-Hua Wu Hosakatte Niranjana Murthy Eun-Joo Hahn Kee-Yoeup Paek |
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Affiliation: | (1) Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, South Korea;(2) Department of Botany, Karnatak University, Dharwad, 580 003, India |
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Abstract: | Adventitious roots of Echinacea purpurea were cultured in airlift bioreactors (20 l, 500 l balloon-type, bubble bioreactors and 1,000 l drum-type bubble bioreactor) using Murashige and Skoog (MS) medium with 2 mg indole butyric acid l−1 and 50 g sucrose l−1 for the production of chichoric acid, chlorogenic acid and caftaric acid. In the 20 l bioreactor (containing 14 l MS medium) a maximum yield of 11 g dry biomass l−1 was achieved after 60 days. However, the amount of total phenolics (57 mg g−1 DW), flavonoids (34 mg g−1 DW) and caffeic acid derivatives (38 mg g−1 DW) were highest after 50 days. Based on these studies, pilot-scale cultures were established and 3.6 kg and 5.1 kg dry biomass were achieved in the 500 l and 1,000 l bioreactors, respectively. The accumulation of 5 mg chlorogenic acid g−1 DW, 22 mg chichoric acid g−1 DW and 4 mg caftaric acids g−1 DW were achieved with adventitious roots grown in 1,000 l bioreactors. |
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Keywords: | Adventitious root cultures Airlift bioreactors Caffeic acid derivatives Echinacea purpurea Large-scale cultures |
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