| 人颈淋巴结淋巴细胞酶超微结构定位与活性研究 |
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| 引用本文： | 蔡玉文.人颈淋巴结淋巴细胞酶超微结构定位与活性研究[J].中国组织化学与细胞化学杂志,1996(3). |
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| 作者姓名： | 蔡玉文 |
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| 作者单位： | 中国医科大学组织学与胚胎学教研室 |
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| 基金项目： | 莫斯科肿瘤研究中心进修课题 |
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| 摘 要： | 应用电镜酶细胞化学方法研究了人颈淋巴结淋巴细胞ＡＴＰ酶、Ｇ－６－Ｐ酶、５’－Ｎａｓｅ的定位与活性。结果：ＡＴＰ酶主要定位在淋巴细胞膜及内质网、线粒体等膜相结构。Ｇ－６－Ｐ酶主要定位在内质网、线粒体等膜相结构。５’－Ｎａｓｅ主要定位在细胞膜外表面，在内质网与线粒体股外表面也可见此酶反应颗粒。３种酶定位准确、颗粒清晰，酶反应特异性强。结果提示应用此法可以检测以上酶活性，对判定机体免疫状态、对临床诊断与治疗具有一定意义。
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| 关 键 词： | 颈淋巴结，淋巴细胞，酶，超微细胞化学 |
A STODY OF ULTRASTRUCTURAL LOCALIZATION AND ACTIVITY OF THE LYMPHOPROTEASE OF THE HUMAN NECK LYMPH NODES |
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| Cat Yuwen, et al.A STODY OF ULTRASTRUCTURAL LOCALIZATION AND ACTIVITY OF THE LYMPHOPROTEASE OF THE HUMAN NECK LYMPH NODES[J].Chinese Journal of Histochemistry and Cytochemistry,1996(3). |
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| Authors: | Cat Yuwen |
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| Abstract: | The author studied localization and activities of ATPase, G-6-Pase, 5'-Nucleotidasein lymphocytes of the human neck lymph nodes by using the electron enzyme cytochemicalmethod. The result showed that the reaction granules of ATPase could be seen clearly beneath the cell membrane and endoplasmic reticulum. Granules of G-6-Pase were mainlyobserved on the surface of endoplasmic reticulum and mitochondria. Reaction granules of 5'-Nucleotidase could be found on the surface of cell membrane, endoplasmic reticulum andmitochondria. All these three enzymes were accurately localized with high sensitivity andstrong specificity.The results suggest that we can test all the enzyme activities in the lymphocytes to determine the patients' immunological condition and provide the basis for clinical diagnosis andtreatment. |
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| Keywords: | Neck lymph node Lymphocyte Enzyme Ultrastructure cytochemistry |
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