| 裂殖壶菌酰基载体蛋白(ACP)基因的克隆与表达 |
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| 引用本文： | 李志平,江贤章,田宝玉,舒正玉,黄建忠. 裂殖壶菌酰基载体蛋白(ACP)基因的克隆与表达[J]. 微生物学通报, 2008, 35(2): 0256-0260 |
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| 作者姓名： | 李志平 江贤章 田宝玉 舒正玉 黄建忠 |
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| 作者单位： | 福建师范大学工业微生物教育部工程研究中心,福州,350108 |
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| 基金项目： | 国家自然科学基金 , 福建省科技厅重大项目 , 福建省发改委资助项目 |
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| 摘 要： | ACP(Acyl carrier protein,酰基载体蛋白)参与高度不饱和脂肪酸的PKS(Polyketide synthase)生物合成途径.从Schizochytrium sp.FJU-512 cDNA文库中获得了ACP基因的cDNA克隆.该序列开放读码框全长429 bp,编码142个氨基酸,等电点为5.04,具有4'-磷酸泛酰巯基乙胺(4'-PP)的结合位点.利用BamH Ⅰ/Hind Ⅲ双酶切,并连接到原核表达载体pET-30a,构建了pET-30a/acp表达载体,转化宿主菌E.coll BL21(DE3),IPTG诱导表达.SDS-PAGE分析表明该蛋白得到高效表达.
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| 关 键 词： | 裂殖壶菌 酰基载体蛋白 二十二碳六烯酸 裂殖壶菌 酰基 载体蛋白 基因 克隆与表达 Gene Protein Carrier Acyl Expression 高效表达 分析表 诱导表达 IPTG 宿主菌 转化 原核表达载体 连接 双酶切 BamH |
| 修稿时间： | 2007-09-06 |
Cloning and Expression of Acyl Carrier Protein Gene from Schizochytrium |
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| LI Zhi-Ping,JIANG Xian-Zhang,TIAN Bao-Yu,SHU Zheng-Yu and HUANG Jian-Zhong. Cloning and Expression of Acyl Carrier Protein Gene from Schizochytrium[J]. Microbiology China, 2008, 35(2): 0256-0260 |
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| Authors: | LI Zhi-Ping JIANG Xian-Zhang TIAN Bao-Yu SHU Zheng-Yu HUANG Jian-Zhong |
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| Affiliation: | Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou 350108;Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou 350108;Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou 350108;Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou 350108;Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou 350108 |
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| Abstract: | |
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| Keywords: | Schizochytrium Acyl Carrier Protein Docosahexaenoic Acid |
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