葡萄TST基因家族鉴定及表达分析

【目的】液泡膜糖转运蛋白（tonoplast sugar transporter，TST或者TMT）是植物发育过程中发挥重要作用的一种糖转运蛋白。为探究该基因家族在葡萄生长发育中的作用，并进一步为阐明TST基因功能提供坚实的基础。【方法】通过同源分析法从葡萄基因组中鉴定出13个TST基因，对基因的结构和编码蛋白质进行生物信息学分析；利用qRT-PCR技术分析‘鄞红’葡萄发育过程中不同组织的TST表达水平，并与不同时期葡萄果肉中可溶性糖含量进行相关性分析。【结果】结果表明：该基因家族分布在6条染色体上，存在3对片段重复和3对串联重复基因；根据系统发育将其分为3个亚家族，各亚族家族成员结构相似；顺式作用元件表明TST基因含有大量与激素、光和胁迫响应相关的顺式作用元件；蛋白质结构均显示该家族由α-螺旋和无规则卷曲组成，各亚族蛋白模型相似；qRT-PCR结果显示，VvTST在不同组织中均有表达，并存在时空表达特异性；对葡萄果肉中基因表达量变化与可溶性糖含量变化进行相关性分析发现，4个VvTST（VIT_18s0001g12560、VIT_18s0122g00850、VIT_04s0023g01860和VIT_03s0038g03940）的表达水平与葡萄果肉中可溶性糖的积累呈现相似趋势。【结论】上述研究结果表明，VvTST可能对葡萄果肉中可溶性糖积累起到至关重要的作用。

Identification and expression analysis of TST gene family in grape

【Objective】 The Tonoplast sugar transporter (TST or TMT) is a crucial sugar transport protein in the developmental trajectory of plants. The study aims to investigate the role of this gene family in the progression of grape growth and development, further provide a solid foundation for elucidation of the function of TST gene. 【Methods】 A total of 13 TST genes were identified from the grape genome using homology analysis technique. Information regarding the gene structure and encoded protein was analyzed using bioinformatics tools. Utilizing the qRT-PCR technique, the VvTST expression level in different grape tissues during ''Yinhong'' grape development was analyzed, and a correlation with soluble sugar content in the grapes at different time points were studied. 【Results】 The study''s results indicate that the gene family is distributed across six chromosomes and contains three segmental repetitions and three tandem repetition genes; according to systematics, it can be classified into three subfamilies, each with members sharing structural similarity; cis regulatory elements suggest that TST genes include ample cis regulatory elements related to phytohormone, light, and stress response; the protein structure reveals that the family is composed of α-helices and irregular coils, and the protein model of each subfamily is similar. The qRT-PCR results indicate that VvTST is expressed in various tissues, possessing spatiotemporal expression specificity. Correlation analysis between changes in gene expression levels in grapes and changes in soluble sugar content in grapes found that theexpression levels of four VvTSTs (VIT_18s0001g12560, VIT_18s0122g00850, VIT_04s0023g01860, and VIT_03s0038g03940) exhibit similar trends with the accumulation of soluble sugars in the grape flesh. 【Conclusion】The aforementioned findings indicate that VvTST may play a vital role in the accumulation of soluble sugars in the flesh of grapes.