血清IL-16、CCL27及TRAIL与多发性硬化病情严重程度的相关性及其预测价值

摘要 目的：探讨血清白细胞介素-16（IL-16）、CC趋化因子配体27（CCL27）及肿瘤坏死因子相关凋亡诱导配体（TRAIL）与多发性硬化病情严重程度的相关性及其预测价值，为多发性硬化的诊断提供理论依据。方法：以2018年1月-2022年10月本院收治的多发性硬化患者72例为研究对象，依据症状严重程度分为急性发作期（n=40）及缓解期（n=32），另选取同期于本院接受体检的健康志愿者65例为对照组。以酶联免疫吸附法（ELISA）测定所有研究对象的血清IL-16、CCL27、TRAIL水平。以Spearman相关性分析血清IL-16、CCL27、TRAIL水平与多发性硬化病情严重程度的相关性，绘制受试者特征曲线（ROC）分析血清IL-16、CCL27、TRAIL水平对多发性硬化病情严重程度的预测价值。结果：研究组的血清IL-16、CCL27、TRAIL水平高于对照组（P<0.05）；急性发作期患者的血清IL-16、CCL27、TRAIL水平高于缓解期患者（P<0.05）。Spearman相关性分析显示，血清IL-16、CCL27、TRAIL水平与多发性硬化的病情严重程度呈正相关（r=0.436，0.461，0.447，P<0.05）。ROC曲线分析结果显示，血清IL-16、CCL27、TRAIL水平联合预测多发性硬化病情严重程度的曲线下面积（AUC）为0.939，显著优于各指标单独评估。结论：血清IL-16、CCL27、TRAIL水平与多发性硬化的发生、发展密切相关，早期检测血清IL-16、CCL27、TRAIL水平对多发性硬化病情具有一定的预测价值，且联合预测的价值更高。

Correlation between Serum IL-16, CCL27 and CCL27 and Severity of Multiple Sclerosis and its Predictive Value

ABSTRACT Objective: To investigate the correlation between serum interleukin-16 (IL-16), CC chemokine ligand 27 (CCL27) and TNF-related apoptosis-inducing ligand (TRAIL) and the severity of multiple sclerosis and its predictive value, and to provide theoretical basis for the diagnosis of MS. Methods: A total of 72 patients with multiple sclerosis admitted to our hospital from January 2018 to October 2022 were selected as the study objects. According to the severity of symptoms, they were divided into acute attack period (n=40) and remission period (n=32), and 65 healthy volunteers who underwent physical examination in our hospital during the same period were selected as the control group. Serum levels of IL-16, CCL27 and TRAIL were determined by enzyme-linked immunosorbent assay (ELISA). Spearman correlation was used to analyze the correlation between serum IL-16, CCL27 and TRAIL levels and the severity of multiple sclerosis, and receiver characteristic curve (ROC) was drawn to analyze the predictive value of serum IL-16, CCL27 and TRAIL levels on the severity of multiple sclerosis. Results: The levels of IL-16, CCL27 and TRAIL in the study group were higher than those in the control group (P<0.05). The levels of IL-16, CCL27 and TRAIL in patients with acute attack period were higher than those in remission period(P<0.05). Spearman correlation analysis showed that serum IL-16, CCL27 and TRAIL levels were positively correlated with the severity of multiple sclerosis (r=0.436, 0.461, 0.447, P<0.05). ROC curve analysis showed that the area under the curve (AUC) of serum IL-16, CCL27 and TRAIL combined to predict the severity of multiple sclerosis was 0.939, which was significantly better than that evaluated by each indicator alone. Conclusion: Serum IL-16, CCL27 and TRAIL levels are closely related to the occurrence and development of multiple sclerosis. Early detection of serum IL-16, CCL27 and TRAIL levels has a certain predictive value for multiple sclerosis, and the combined prediction value is higher.