On-line measurement of culture fluorescence during cultivation of Methylomonas mucosa

Summary This paper reports on the measurement of NAD(P)H dependent culture fluorescence during the batch culture of Methylomonas mucosa, a potential polysaccharide producer. The results obtained reveal that there is a high correlation between the fluorescence level and the cell concentration. The logistic equation was also found to be applicable for representing the biomass concentration data during the course of fermentation. A model was then presented for predicting both the biomass concentration and the culture fluorescence with high fidelity.