| 分子标记技术在玉米品种分析中的初步应用 |
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| 引用本文： | 马纯艳,郑莹,李玥莹,陶思源,徐昕.分子标记技术在玉米品种分析中的初步应用[J].生物技术,2005,15(3):57-58. |
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| 作者姓名： | 马纯艳 郑莹 李玥莹 陶思源 徐昕 |
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| 作者单位： | 沈阳师范大学生物系,辽宁,沈阳,110034 |
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| 基金项目： | 沈阳市科技局国际合作项目(“半干旱作物的抗虫分子标记”,1041053-6-04)
辽宁省科委项目(“玉米丝黑穗病功能基因的分子标记及其克隆研究”,20021022)
辽宁省教育厅项目资助(20211202)
沈阳师范大学实验中心主任基金项目资助 |
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| 摘 要： | 以玉米沈丹16、沈丹2100的可见叶片为材料，采用CTAB-Ⅱ方法提取玉米基因组DNA，然后应用RAPD分子标记技术对基因组DNA进行多态性扩增。结果是：从RAPD反应所用的40个随机引物中筛选出11个适宜引物，共扩增出63条谱带，其中14条为差异性谱带，其余引物没有扩增出谱带，被淘汰；此次实验的RAPD反应系统虽然较成功，但不是最佳的，今后要进一步优化。
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| 关 键 词： | 玉米 CTAB 分子标记 RAPD |
Primary Application of Molecular Marker Technique in Analyzing the Culfivars of Maize |
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| MA Chun-yan,ZHENG Ying,LI Yue-ying,TAO Si-yuan,XU Xin.Primary Application of Molecular Marker Technique in Analyzing the Culfivars of Maize[J].Biotechnology,2005,15(3):57-58. |
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| Authors: | MA Chun-yan ZHENG Ying LI Yue-ying TAO Si-yuan XU Xin |
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| Institution: | MA Chun-yan,ZHENG Ying,LI Yue-ying *,TAO Si-yuan,XU Xin |
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| Abstract: | Leaves of two cultivars of corn (Shendan 16,Shendan 2100)were used in this study .Total DNA was extracted using one method, which was Cetyl Trimethy Lammonium Bromide(CTAB).Then total DNA was amplified by the Random Amplified Dolymorphism DNA(RAPD)technology. The results showed that: With RAPD, 11 random sequence primers were selected by RAPD technology. And 63 bands of DNA were amplified, and 14 different bands were amplified by 6 primers, the others did not generate polymorphism bands, being eliminated; In this study, this RAPD system condition can be successfully used to amplify. But the RAPD system condition was not the optimum. So the reaction condition should be studied and optimum. |
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| Keywords: | corn CTAB molecular markers random amplified polymorphism DNA (RAPD) |
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