Spatial analysis of intranuclear human repetitive DNA regions by in situ hybridization and digital fluorescence microscopy |
| |
Authors: | H van Dekken and R Hulspas |
| |
Institution: | (1) Department of Pathology, Erasmus University Rotterdam, 3000 DR Rotterdam, PO Box 1738, Rijswijk, The Netherlands;(2) Department of Molecular Pathology, ITRI-TNO, Rijswijk, The Netherlands |
| |
Abstract: | Summary Non-isotopic (fluorescent) in situ hybridization has established itself as a useful technique for the localization of DNA sequences in both metaphase and interphase cells. The rapid development of digital fluorescence microscopy, especially confocal microscopy, has become a powerful aid for the evaluation of the hybridization results in cytogenetic and cell biological applications. In this review we will demonstrate the utility of these methodologies for the three-dimensional visualization and analysis of chromosome-specific (peri)centromeric repetitive DNA sequences within the intranuclear structure of human cells and cell lines. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|