Genetic Transformation in Haemophilus parainfluenzae Clinical Isolates |
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Authors: | Rosa C Gromkova Tanya C Mottalini Michael G Dove |
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Institution: | (1) Department of Medical Microbiology, University of Pretoria, PO Box 2034, Pretoria 0001, South Africa , ZA |
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Abstract: | Haemophilus parainfluenzae isolates recovered from patients with respiratory diseases were studied for their ability to undergo genetic transformation
by isogenic DNA. Two chromosomal markers, streptomycin resistance and nalidixic acid resistance, were tested for transformation
efficiencies in H. parainfluenzae recipients from three biotypes. Most efficient in transformation was biotype II, followed by biotype I, while biotype III
was nontransformable. Lack of transformation was not owing to poor donor activity of DNA, but to inability of the cells to
develop competence. Strains that formed clumps in liquid media were nontransformable. Since the transformable biotype II is
one of the prevalent biotypes world wide, one can speculate that DNA transformation probably plays a major role in the spread
of drug resistance in H. parainfluenzae.
Received: 9 December 1997 / Accepted: 26 February 1998 |
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