Detection ofMycoplasmain Avian Live Virus Vaccines by Polymerase Chain Reaction |
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| Authors: | Akemi Kojima Toshio Takahashi Mayumi Kijima Yasuaki Ogikubo Makoto Nishimura Shinzo Nishimura Ryo Harasawa Yutaka Tamura |
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| Institution: | aNational Veterinary Assay Laboratory, Kokubunji, Tokyo 185, Japan;bDainippon Pharmaceutical Co., Ltd. Suita, Osaka 564, Japan;cAnimal Center for Biomedical Research, Faculty of Medicine, The University of Tokyo, Tokyo 113, Japan |
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| Abstract: | The polymerase chain reaction (PCR) was evaluated to detect mycoplasma contamination of avian live virus vaccines. The specificity of the primers showed that 34 strains belonging to nine species of avian mycoplasma DNA could be detected. The sensitivity of PCR to detect mycoplasma DNA was 100·2colony forming units (cfu) ofMycoplasma synoviaeand 100·7cfu ofMycoplasma gallisepticum. WhenM. synoviaeandM. gallisepticumwere spiked into several avian live virus vaccines, PCR gave a positive reaction except for the avian pox and the avian encephalomyelitis vaccines which were prepared from organ homogenates. Short-term incubation of avian encephalomyelitis vaccine improved the sensitivity of PCR to detect bothM. synoviaeandM. gallisepticum. Therefore, PCR, combined with the short-term incubation, were shown to be most effective in detecting mycoplasma contamination in all of avian live virus vaccines. |
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