Immunological Detection of Hymenopteran Parasitism inHelicoverpa zeaandHeliothis virescens |
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| Authors: | Melissa K. Stuart Matthew H. Greenstone |
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| Affiliation: | aDepartment of Microbiology and Immunology, Kirksville College of Osteopathic Medicine, Kirksville, Missouri, 63501;bU.S. Department of Agriculture, Agricultural Research Service, Plant Science and Water Conservation Research Laboratory, Stillwater, Oklahoma, 74075 |
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| Abstract: | We have developed a monoclonal antibody that recognizes the larval and adult stages ofMicroplitis croceipes(Cresson) (Hymenoptera: Braconidae) andCotesia marginiventris(Cresson) (Hymenoptera: Braconidae), two principal endoparasitoids of the polyphagous pestsHelicoverpa zea(Boddie) (Lepidoptera: Noctuidae) andHeliothis virescens(F.) (Lepidoptera: Noctuidae). The antibody has been incorporated into an indirect enzyme-linked immunosorbent assay that can be used to distinguish parasitized from unparasitized pests. The antibody cross-reacts with several different hymenopteran parasitoids, but not with any of the noctuid pests we have assayed. An immunoassay based on a broadly reactive antibody such as this one enables comprehensive detection of hymenopteran parasitism with a single antibody reagent. |
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| Keywords: | Microplitis croceipes Cotesia marginiventris Helicoverpa zea Heliothis virescens cotton bollworm tobacco budworm endoparasitism monoclonal antibody immunoassay |
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