A novel isoform of ATPase c subunit from pearl millet that is differentially regulated in response to salinity and calcium |
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Authors: | Wricha Tyagi SnehLata Singla-Pareek Suresh Nair M K Reddy S K Sopory |
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Institution: | (1) International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India |
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Abstract: | Vacuolar ATPases help in maintaining the pH of the vacuoles and thereby play a crucial role in the functioning of vacuolar
sodium-proton antiporter. Though the various subunits that make V1 and V0 sector have been reported in plants their regulation is not understood completely. We have cloned three different isoforms
of vacuolar ATPase subunit c (VHA-c) from Pennisetum glaucum with homologies among themselves varying from 38% to ∼73% at the nucleic acid level. Using real-time PCR approach we have
shown that the three isoforms are regulated in a tissue-specific manner under salinity stress. While isoform III is constitutively
expressed in roots and shoots and does not respond to stress, isoform I is upregulated under stress. Isoform II is expressed
mainly in roots; however, under salinity stress its expression is downregulated in roots and upregulated in shoots. Tissue
specific expression under salinity stress of isoform II was also seen after exogenous application of calcium. This study for
the first time shows the presence of three isoforms of PgVHA-c and their differential regulation during plant development, and also under abiotic stress. |
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Keywords: | Abiotic stress Differential regulation Pennisetum Real-time PCR Vacuolar ATPase |
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