轮状病毒基因重配LH9株(G4型)传代稳定性研究

对兰州生物制品研究所自行构建的轮状病毒基因重配株G4型LH9株在Vero细胞中传代的稳定性进行研究。将基因重配LH9毒株接种于Vero细胞上从7代传至17代,经电镜检查、病毒滴度测定、病毒基因组RNA电泳图谱分析、基因序列测定分析、轮状病毒型别鉴定及其它相关检定,结果均符合药典要求。证实该毒株为A群G4P〔12〕型轮状病毒,在Vero细胞传代中具有良好的稳定性,为疫苗研制提供安全有效的依据。

Stability of a LLR-Hochi reassortant rotavirus vaccine strains LH9

To evaluate the stability of a LLR-Hochi Reassortant rotavirus vaccine strains LH9 from Lanzhou Institute of Biological Products.The LH9 strain generated from Vero cell was tested by electron microscopy analysis,virus titration,RNA electrophoretic pattern,DNA sequencing,the determination of serotype and the examination of biology.The experimental results showed that LH9 strain belonged to group A rotaviruss,The hereditary character of LH9 is comparatively stable and there is no difference among the different generations,The results obtained provide significant basis for the development of vaccine.