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Separation of the minimal replication region of the F plasmid into a replication origin segment and a trans-acting segment
Authors:A Tolun  D R Helinski
Institution:(1) University of California, 92093 San Diego, La Jolla, California, USA;(2) Present address: Biology Department, Bo gaziçi University, PK2, Bebek, Istanbul, Turkey
Abstract:Summary An analysis was carried out on the replication functions within a 2.3 kilobase (kb) segment of the F plasmid which contains an origin (ori S) of replication and is capable of autonomous replication inEscherichia coli. Two separable regions were delineated for this segment: an origin region of approximately 1,140 bp in length and a segment of approximately 1,400 bp that functionsin trans to support replication of the origin region. The trans-acting segment is functional as part of an F replicon or when inserted into theE. coli chromosome. A prominent feature of the trans-acting segment is a coding sequence for a 29 K protein (Murotsu et al. 1981).
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