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High-level expression of a viscotoxin in Arabidopsis thaliana gives enhanced resistance against Plasmodiophora brassicae |
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| Authors: | Holtorf Sönke Ludwig-Müller Jutta Apel Klaus Bohlmann Holger |
| Institution: | (1) Swiss Federal Institute of Technology (ETH), Institute of Plant Sciences, ETH-Zentrum, Universitätstrasse 2, LFW D.58, 8092 Zürich, Switzerland;(2) Johann Wolfgang Goethe-Universität, Botanisches Institut, Postfach 111932, 60054 Frankfurt/Main, Germany;(3) Present address: European Patent Office, Directorate 1.2.12, Chemistry, 2280 HV Rijswijk (ZH), Netherlands |
| Abstract: | Viscotoxins are a group of toxic thionins found in several mistletoe species. The constitutive CaMV- promoter was used to drive the expression of the viscotoxin A3 cDNA from Viscum album in transgenic Arabidopsis thaliana C24. Lines with high viscotoxin A3 levels in all parts of the plant were selected and tested for resistance against the clubroot pathogen Plasmodiophora brassicae. The transgenic lines were more resistant to infection by this pathogen than the parental line. |
| Keywords: | plant defence thionins cysteine-rich antimicrobial |
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