Gibberellin metabolism in excised lettuce hypocotyls: Evidence for the formation of gibberellin A1 glucosyl conjugates |
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Authors: | John L. Stoddart Russell L. Jones |
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Affiliation: | (1) Welsh Plant Breeding Station, SY23 3EB Aberystwyth, U.K.;(2) Department of Botany, University of California, 94720 Berkeley, CA, USA |
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Abstract: | The properties of the water-soluble metabolites of [3H]gibberellin A1 ([3H]GA1) from lettuce (Lactuca sativa L.) hypocotyls were compared with those of authentic samples of gibberellin (GA) glucosyl esters and ethers. Partitioning against l-butanol at high and low pH was not an efficient method of differentiating between ester and ether conjugates of GA1 or GA3. Extraction into l-butanol at pH 2.5 was, however, useful as a group purification step. Gel-filtration on acrylamide indicated a mean molecular weight of ca. 600 for the polar material and high-voltage electrophoresis separated two compounds (LH 1 and LH 2) with differing charge properties. Both metabolites incorporated 14C from glucose and 3H from GA1. Subsequent enzymatic hydrolysis of LH 1 released material with identical properties to [14C]glucose together with a second uncharacterised component. Feeding with [3H]GA1 methyl ester greatly reduced the formation of LH 1 but not LH 2. The metabolites were provisionally identified as GA1-glucosyl ester (LH 1) and GA1-glucosyl ether (LH 2).Abbreviations GA gibberellin - LH1 GA3-glucosyl ester - LH2 GA1-glucosyl ether - HVE high voltage paper electrophoresis - TLC thin-layer chromatography |
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Keywords: | Conjugates Gibberellin Lactuca Metabolism |
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