Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in <Emphasis Type="Italic">Klebsiella oxytoca</Emphasis> |
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Authors: | Xiao-Jun Ji He Huang Shuang Li Jun Du Min Lian |
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Institution: | (1) College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing, 210009, P.R. China |
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Abstract: | Klebsiella oxytoca mutants were isolated from the wild type strain ME-303 after mutagenesis with UV coupled with diethyl sulfate and the following
modified proton suicide method. By analyzing the activities of lactate dehydrogenase and phosphotransacetylase involved in
lactic and acetic acid formation pathways and batch fermentation, one mutant, ME-UD-3, was isolated that produced 7.8% more
2,3-butanediol than ME-303 with the corresponding byproducts of lactic and acetic acid decreased by 88% and 92%, respectively. |
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Keywords: | 2 3-Butanediol Klebsiella oxytoca Proton-suicide method |
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