Germination of trinucleated pollen: formulation of a new medium for Capsella bursa-pastoris |
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Authors: | N Leduc M Monnier G C Douglas |
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Institution: | (1) Laboratoire d'Histophysiologie Vegetale, Université Pierre et Marie Curie, 12, rue Cuvier, F-75005 Paris, France;(2) The Agriculture and Food Development Authority, Kinsealy Research Centre, Malahide Road, 17 Dublin, Ireland;(3) Laboratoire d'Histophysiologie Vegetale, Université Pierre et Marie Curie, 12, rue Cuvier, F-75005 Paris, France |
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Abstract: | Summary In Brewbaker and Kwack's medium (BK) only 16% of the pollen grains germinated, and these produced pollen tubes having a maximum length of 25 m. With a solution based on Monnier's medium 47% germination and 160- mlong pollen tubes were observed. Calcium was shown to be essential for germination; the optimal concentration was 880 mg/l calcium chloride. The optimal concentrations of magnesium sulphate and boric acid were 360 and 50 mg/l, respectively. Germination at pH 4.0 but also pH 8.0 and the presence of vitamins B1 and B6 (1 mg/l each) were stimulatory. Polyethylene glycol (PEG) was superior to sucrose as an osmoticum and germination and tube length were significantly improved using PEG 4000 at a concentration of 120 g/l (0.03 M). Equimolar concentrations of PEG 400 and PEG 600 gave inferior results. Combining PEG with sucrose in the medium did not improve germination or increase tube length. |
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Keywords: | Trinucleate pollen In vitro germination Polyethylene glycol |
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