Karyotyping of the narrow-leafed lupin (Lupinus angustifolius L.) by using FISH, PRINS and computer measurements of chromosomes |
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| Authors: | A Kaczmarek B Naganowska B Wolko |
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| Institution: | (1) School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, Perth, WA, 6009, Australia;(2) Australian Centre for Necrotrophic Fungal Pathogens, WA State Agricultural Biotechnology Centre, Division of Health Sciences, Murdoch University, Perth, WA, 6150, Australia;(3) Centre for Bioinformatics and Biological Computing, Murdoch University, Perth, WA, 6150, Australia;(4) WA State Agricultural Biotechnology Centre, School of Biological Sciences and Biotechnology, Division of Science and Engineering, Murdoch University, Perth, WA, 6150, Australia;(5) Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland; |
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| Abstract: | BAC (bacterial artificial chromosome) clones from the genomic BAC library of the narrow-leafed lupin (Lupinus angustifolius) were used for cytogenetic mapping of mitotic metaphase chromosomes of that species by the BAC-FISH technique. Location of
the clones, together with cytogenetic markers localised earlier by FISH (fluorescencein situ hybridisation) and PRINS (primedin situ DNA labelling), was combined with computer-aided chromosome measurements, to construct the first idiogram of the narrow-leafed
lupin. The chromosomes are meta- or submetacentric; the mean absolute chromosome lengths range from 1.9 μm to 3.8 μm, and
mean relative lengths from 1.6% to 3.3%. Data concerning linkage of resistance to 2 fungal pathogens as well as assignment
of the second linkage group to the appropriate chromosome are given for the first time. |
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