Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae |
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| Authors: | Marie-Ange Teste Manon Duquenne Jean M Fran?ois Jean-Luc Parrou |
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| Affiliation: | (1) CNRS, UMR5504, F-31400 Toulouse, France;(2) INRA, UMR792 Ing?nierie des Syst?mes Biologiques et des Proc?d?s, F-31400 Toulouse, France;(3) Universit? de Toulouse; INSA, UPS, INP; LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France;(4) Unit? des Bact?ries Lactiques et pathog?nes Opportunistes, INRA - Centre de Recherche de Jouy-en-Josas, Domaine de Vilvert, 78352 Jouy-en Josas, France |
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| Abstract: | Background Real-time RT-PCR is the recommended method for quantitative gene expression analysis. A compulsory step is the selection of good reference genes for normalization. A few genes often referred to as HouseKeeping Genes (HSK), such as ACT1, RDN18 or PDA1 are among the most commonly used, as their expression is assumed to remain unchanged over a wide range of conditions. Since this assumption is very unlikely, a geometric averaging of multiple, carefully selected internal control genes is now strongly recommended for normalization to avoid this problem of expression variation of single reference genes. The aim of this work was to search for a set of reference genes for reliable gene expression analysis in Saccharomyces cerevisiae. |
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