泰和乌骨鸡的核型与带型研究 |
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作者姓名: | 陈国宏 刘莉 张学余 李碧春 吴信生 徐琪 |
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作者单位: | 1.扬州大学畜牧兽医学院动科系,江苏 扬州 225009;2.江苏省家禽科学研究所,江苏 扬州 2250031.Dept of Anim Sci,Anim Sci and Med Coll,Yangzhou Uni,Yangzhou 225009,China;2.Jiangsu Provincial Institute of Poultry Science,Yangzhou 225003,China |
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基金项目: | 国家高新技术发展计划项目(863—2001AA243083),国家自然科学基金(30170673) |
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摘 要: | 采用外周血淋巴细胞培养--空气干燥法,对泰和乌骨鸡染色体核型和带型进行了研究。结果表明:泰和乌骨鸡体细胞染色体数目2n=78,染色体基本臂数AF=90,1、9号染色体及Z、W性染色体为中央着丝粒(m)染色体,2、4、7号染色体为亚中央着丝粒(sm)染色体,3、6、8、10号染色体为端着丝粒(t)染色体。G带研究表明:前10对大型染色体可分为29个区,190条带。C带处理发现,所有母鸡分裂相中W性染色体都出现C带并整条深染。Ag-NORs研究发现:Ag-NORs常定位于1、2号常染色体短臂和Z性染色体短臂端部;Ag-NORs数目分布范围为1~6;平均每个细胞的Ag-NORs数在雌、雄鸡中分别为2.94和2.96。Abstract:This study made the chromosome slides of Taihe Silkies by the peripheral blood lymphocyte culture-drying method,and analyzed Taihe Silkies karyotype and band pattern.The results are as follow:The diploid chromosome number of Taihe Silkies was 2n=78,the basic number of chromosome arms was AF=90 and the sex chromosome type was ZZ(♂)/ZW(♀).According to the measured relative length,arm ratio and centromeric index,the first 10 pairs of macro-chromosomes are described as follows:No.1 ,9 and Z,W chromosomes were metacentrics,No.2,4,7 were submetacentrics,and No.3,6,8,10 were telocentrics.Studies on Taihe Silkies′ G-band showed that the first 10 pairs of macro-chromosomes can be divided into 29 zones and 190 bands.Being treated by C-banding technique,a totally dark-stained and easily indentified W-chromosome always showed up in the female metaphase configurations.Ag-NORs were located in the short arms′ telomere of No.1,2 euchromosomes and Z sexchromosome,the Ag-NORs number varied from 1-6.-NORs
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关 键 词: | 测序 基因组 日本扁柏 微卫星 Ag-NORs Key words C带 G带 泰和乌骨鸡 核型 |
文章编号: | 0253-9772(2003)04-0401-08 |
修稿时间: | 2002-07-03 |
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