| 乳糖诱导木聚糖酶基因在大肠杆菌中的可溶性表达 |
| |
| 引用本文： | 周晨妍,符丹丹,王卫杰,曹利军,丰慧根.乳糖诱导木聚糖酶基因在大肠杆菌中的可溶性表达[J].工业微生物,2010,40(4):8-12. |
| |
| 作者姓名： | 周晨妍 符丹丹 王卫杰 曹利军 丰慧根 |
| |
| 作者单位： | 1. 新乡医学院生命科学技术系,河南省医学遗传学与分子靶向药物高校重点实验室培育基地,河南新乡,453003
2. 河南科技大学食品与生物工程学院,河南洛阳,471003 |
| |
| 基金项目： | 新乡医学院高学历人才支持计划基金 |
| |
| 摘 要： | 以构建好的大肠杆菌工程菌BL21(DE3)/xylanase为研究对象,研究了以IPTG和乳糖作为诱导剂时重组蛋白的表达规律。在摇瓶发酵条件下研究了诱导剂浓度、诱导时机、诱导培养时间和诱导培养温度对目标蛋白表达的影响。实验结果表明,乳糖作为诱导剂时,重组菌产酶活力33.9 U/mg略高于IPTG作为诱导剂时重组菌产酶活力28.10 U/mg,这为乳糖作为诱导剂应用于重组大肠杆菌生产木聚糖酶提供了参考依据。
|
| 关 键 词： | 木聚糖酶 乳糖 诱导表达 优化 |
Soluble expression of recombinant xylanase in E. coli using lactose as inducer |
| |
| ZHOU Chen-yan,FU Dan-dan,WANG Wei-jie,CAO Li-jun,FENG Hui-gen.Soluble expression of recombinant xylanase in E. coli using lactose as inducer[J].Industrial Microbiology,2010,40(4):8-12. |
| |
| Authors: | ZHOU Chen-yan FU Dan-dan WANG Wei-jie CAO Li-jun FENG Hui-gen |
| |
| Institution: | 1. The Key Laboratory of Medical Genetics and Molecular Targeting Drugs, Henan Pmvinee, Department of Life Scienee and Technology, Xinxiang Medical University, Xinxiang 453003, China; 2. College of Food and Biological Engineering, Henan University of Science and Technology, Luoyang, Henan 471003, China) |
| |
| Abstract: | In order to express recombinant xylanase in E. coli BL21（DE3）/xylanase in a soluble form using lactose and IPTG as inducers, the conditions for the expression were optimized. The influences of culture conditions such as concentration of the inducer, the time of culture before induction, the time and the temperature of induction culture on the expression of the recombinant protein of E. coli BL 21 （DE3）]xylanase were investigated in detail. The activity of xylanase induced by lactose was higher than that induced by IPTG and the expression level of recombinant protein induced by lactose was basically the same as that induced by IPTG. The result indicated that lactose could be used as a promising inducer in the production of recombined xylanase. |
| |
| Keywords: | xylanase lactose E coli induction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
