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Abundance and diversity of archaeal accA gene in hot springs in Yunnan Province, China |
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| Authors: | Zhao-Qi Song Li Wang Feng-Ping Wang Hong-Chen Jiang Jin-Quan Chen En-Min Zhou Feng Liang Xiang Xiao Wen-Jun Li |
| Institution: | 1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, China 2. Engineering Technology Research Center of Biomass Degradation and Gasification, University of Henan Province, Shangqiu Normal University, Shangqiu, 476000, China 3. Key Laboratory of MOE for Microbial Metabolism and School of Life Sciences and Biotechnology, State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China 4. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, China 5. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, ür?mqi, 830011, China |
| Abstract: | It has been suggested that archaea carrying the accA gene, encoding the alpha subunit of the acetyl CoA carboxylase, autotrophically fix CO2 using the 3-hydroxypropionate/4-hydroxybutyrate pathway in low-temperature environments (e.g., soils, oceans). However, little new information has come to light regarding the occurrence of archaeal accA genes in high-temperature ecosystems. In this study, we investigated the abundance and diversity of archaeal accA gene in hot springs in Yunnan Province, China, using DNA- and RNA-based phylogenetic analyses and quantitative polymerase chain reaction. The results showed that archaeal accA genes were present and expressed in the investigated Yunnan hot springs with a wide range of temperatures (66–96 °C) and pH (4.3–9.0). The majority of the amplified archaeal accA gene sequences were affiliated with the ThAOA/HWCG III thermophilic ammonia-oxidizing archaea (AOA)/hot water crenarchaeotic group III]. The archaeal accA gene abundance was very close to that of AOA amoA gene, encoding the alpha subunit of ammonia monooxygenase. These data suggest that AOA in terrestrial hot springs might acquire energy from ammonia oxidation coupled with CO2 fixation using the 3-hydroxypropionate/4-hydroxybutyrate pathway. |
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