Activation of membrane phospholipase C by vasopressin. A requirement for guanyl nucleotides |
| |
Authors: | G Guillon M N Balestre B Mouillac G Devilliers |
| |
Affiliation: | Centre CNRS-INSERM de Pharmacologie-Endocrinologie, Rue de la Cardonille, BP 5055, 34100 Montpellier, France |
| |
Abstract: | Vasopressin stimulates the liberation of labelled inositol phosphate in partially purified plasma membranes prepared from myo-[3H]inositol prelabelled WRK1 cells. This stimulatory effect was very rapid (165% stimulation of inositol trisphosphate accumulation after a 10 s incubation period in the presence of 1 microM vasopressin), concentration dependent (EC50 = 12 nM) and was abolished by an antagonist of the vasopressor response to vasopressin. GTP, even at high concentrations (0.1 mM), did not increase inositol phosphate release: it was found to be absolutely necessary for hormonal stimulation of phospholipase C activity. Non-hydrolysable analogues of GTP may also stimulate this enzyme activity. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|