|
|||||
|
|
Inhibitory effects of glutathione on dengue virus production |
|
| Authors: | Yanping Tian Wen Jiang Na Gao Wei Chen Deshan Zhou |
| Institution: | a Department of Histology and Embryology, School of Basic Medicine, Third Military Medical University, Chongqing 400038, PR China b Department of Laboratory Animal Sciences, School of Basic Medicine, Third Military Medical University, Chongqing 400038, PR China c Department of Microbiology, School of Basic Medicine, Third Military Medical University, Chongqing 400038, PR China d Department of Microbiology, School of Basic Medical Sciences, Capital University of Medical Sciences, Beijing 100069, PR China e Department of Histology and Embryology, School of Basic Medical Sciences, Capital University of Medical Sciences, Beijing 100069, PR China |
| Abstract: | Reduced glutathione (GSH) is the most powerful intracellular antioxidant and also involved in viral infections. The pathogenesis of dengue virus (DV) infection has not been completely clarified. This study investigated the relationship between DV serotype 2 (DV2) infections and host intracellular GSH content. Results showed infection with DV2 resulted in a decrease in intracellular GSH, which caused NF-κB activation and increased DV2 production. Supplemental GSH significantly inhibited activation of NF-κB, resulting in a decreased production of DV2 in HepG2 cells. Furthermore, high activity of NF-κB and increased production of DV2 was observed in HepG2 cells treated with buthionine sulfoximine (BSO), an inhibitor of GSH synthesis. In conclusion, DV2 infection could reduce host intracellular GSH concentration and benefited from this process. Supplemental GSH could inhibit viral production, indicating GSH might be valuable in the prevention and treatment of DV2 infection. |
| Keywords: | Dengue virus Redox stage GSH NF-κB |
| 本文献已被 ScienceDirect 等数据库收录！ | |