The viscoelastic properties of microvilli are dependent upon the cell-surface molecule |
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Authors: | Johanne L. Python Jeremy H. Snook William H. Guilford |
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Affiliation: | a Department of Biomedical Engineering, University of Virginia, Box 800759, Charlottesville, VA 22908, USA b Department of Mechanical Engineering, University of California at Berkeley, Berkeley, CA 94702, USA |
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Abstract: | We studied at nanometer resolution the viscoelastic properties of microvilli and tethers pulled from myelogenous cells via P-selectin glycoprotein ligand 1 (PSGL-1) and found that in contrast to pure membrane tethers, the viscoelastic properties of microvillus deformations are dependent upon the cell-surface molecule through which load is applied. A laser trap and polymer bead coated with anti-PSGL-1 (KPL-1) were used to apply step loads to microvilli. The lengthening of the microvillus in response to the induced step loads was fitted with a viscoelastic model. The quasi-steady state force on the microvillus at any given length was approximately fourfold lower in cells treated with cytochalasin D or when pulled with concanavalin A-coated rather than KPL-1-coated beads. These data suggest that associations between PSGL-1 and the underlying actin cytoskeleton significantly affect the early stages of leukocyte deformation under flow. |
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Keywords: | PSGL-1 Concanavalin A Actin cytoskeleton Laser traps Viscoelastic models |
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