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Human selenophosphate synthetase 1 has five splice variants with unique interactions, subcellular localizations and expression patterns |
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| Authors: | Jin Young Kim Myoung Sup Shim Xue-Ming Xu Dolph L Hatfield |
| Institution: | a Laboratory of Molecular Genetics and Genomics, School of Biological Sciences, Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Republic of Korea b Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul National University, Seoul 151-742, Republic of Korea c Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA |
| Abstract: | Selenophosphate synthetase 1 (SPS1) is an essential cellular gene in higher eukaryotes. Five alternative splice variants of human SPS1 (major type, ΔE2, ΔE8, +E9, +E9a) were identified wherein +E9 and +E9a make the same protein. The major type was localized in both the nuclear and plasma membranes, and the others in the cytoplasm. All variants form homodimers, and in addition, the major type forms a heterodimer with ΔE2, and ΔE8 with +E9. The level of expression of each splice variant was different in various cell lines. The expression of each alternative splice variant was regulated during the cell cycle. The levels of the major type and ΔE8 were gradually increased until G2/M phase and then gradually decreased. ΔE2 expression peaked at mid-S phase and then gradually decreased. However, +E9/+E9a expression decreased gradually after cell cycle arrest. The possible involvement of SPS1 splice variants in cell cycle regulation is discussed. |
| Keywords: | AA antibiotic-antimycotic CT cycle-threshold FACS fluorescent associated cell sorter FBS fetal bovine serum HA haemagglutinin MT major type PI propidium iodide ROS reactive oxygen species SPS selenophosphate synthetase Sec selenocysteine SecS Sec synthase |
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