|
|||||
|
|
DNA-PK/Ku complex binds to latency-associated nuclear antigen and negatively regulates Kaposi’s sarcoma-associated herpesvirus latent replication |
|
| Authors: | Seho Cha Chunghun Lim Jae Young Lee Junsoo Park Taegun Seo |
| Institution: | a Department of Life Science, Dongguk Univ-Seoul, Seoul 100-715, South Korea b Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea c Department of Life Science, Kyungwon University, Seongnam-Si, Kyeonggi-Do 461-701, South Korea d Division of Biological Science and Technology, Yonsei University, Wonju 220-100, South Korea |
| Abstract: | During latent infection, latency-associated nuclear antigen (LANA) of Kaposi’s sarcoma-associated herpesvirus (KSHV) plays important roles in episomal persistence and replication. Several host factors are associated with KSHV latent replication. Here, we show that the catalytic subunit of DNA protein kinase (DNA-PKcs), Ku70, and Ku86 bind the N-terminal region of LANA. LANA was phosphorylated by DNA-PK and overexpression of Ku70, but not Ku86, impaired transient replication. The efficiency of transient replication was significantly increased in the HCT116 (Ku86 +/−) cell line, compared to the HCT116 (Ku86 +/+) cell line, suggesting that the DNA-PK/Ku complex negatively regulates KSHV latent replication. |
| Keywords: | LANA KSHV DNA-PK Ku Latent replication |
| 本文献已被 ScienceDirect 等数据库收录！ | |