Repressive BMP2 gene regulatory elements near the BMP2 promoter |
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| Authors: | Shan Jiang Ronald L. Chandler Douglas P. Mortlock |
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| Affiliation: | a Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry (UMDNJ), New Jersey Medical School (NJMS), Newark, NJ, USA
b Department of Molecular Physiology and Biophysics, Center for Human Genetics Research, Vanderbilt University School of Medicine, Nashville, TN, USA |
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| Abstract: | The level of bone morphogenetic protein 2 (BMP2) profoundly influences essential cell behaviors such as proliferation, differentiation, apoptosis, and migration. The spatial and temporal pattern of BMP2 synthesis, particular in diverse embryonic cells, is highly varied and dynamic. We have identified GC-rich sequences within the BMP2 promoter region that strongly repress gene expression. These elements block the activity of a highly conserved, osteoblast enhancer in response to FGF2 treatment. Both positive and negative gene regulatory elements control BMP2 synthesis. Detecting and mapping the repressive motifs is essential because they impede the identification of developmentally regulated enhancers necessary for normal BMP2 patterns and concentration. |
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| Keywords: | BMP, bone morphogenetic protein FGF2, fibroblast growth factor nt, nucleotides kb, kilobases RAR, retinoic acid receptor RA, retinoic acid EC, embryonal carcinoma BAC, bacterial artificial chromosome |
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