Functional expression of 5-HT2A receptor in osteoblastic MC3T3-E1 cells |
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Authors: | Takao Hirai |
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Affiliation: | Department of Molecular Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, 1 Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan |
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Abstract: | In the previous study, we reported the gene expression for proteins related to the function of 5-hydroxytryptamine (5-HT, serotonin) and elucidated the expression patterns of 5-HT2 receptor subtypes in mouse osteoblasts. In the present study, we evaluated the possible involvement of 5-HT receptor subtypes and its inactivation system in MC3T3-E1 cells, an osteoblast cell line. DOI, a 5-HT2A and 5-HT2C receptor selective agonist, as well as 5-HT concentration-dependently increased proliferative activities of MC3T3-E1 cells in their premature period. This effect of 5-HT on cell proliferation were inhibited by ketanserin, a 5-HT2A receptor specific antagonist. Moreover, both DOI-induced cell proliferation and phosphorylation of ERK1 and 2 proteins were inhibited by PD98059 and U0126, selective inhibitors of MEK in a concentration-dependent manner. Furthermore, treatment with fluoxetine, a 5-HT specific re-uptake inhibitor which inactivate the function of extracellular 5-HT, significantly increased the proliferative activities of MC3T3-E1 cells in a concentration-dependent manner. Our data indicate that 5-HT fill the role for proliferation of osteoblast cells in their premature period. Notably, 5-HT2A receptor may be functionally expressed to regulate mechanisms underlying osteoblast cell proliferation, at least in part, through activation of ERK/MAPK pathways in MC3T3-E1 cells. |
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Keywords: | 5-HT, 5-hydroxytryptamine 5-HTT, 5-HT transporter α-MEM, α-modification of minimum essential medium DIC, days in culture DOI, (± )-1-(2,5-di-methoxy-4-iodophenyl-2) aminopropane ERK, extracellular signal-regulated-kinase FBS, fetal bovine serum GPCR, G-protein coupled receptor MAPK, mitogen-activated protein kinase MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide RT-PCR, reverse transcription polymerase chain reaction SSRI, selective serotonin re-uptake inhibitors |
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