An expressed sequence tag (EST) library from developing fruits of an Hawaiian endemic mint (Stenogyne rugosa, Lamiaceae): characterization and microsatellite markers |
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Authors: | Charlotte Lindqvist Anne-Cathrine Scheen Mi-Jeong Yoo Paris Grey David G Oppenheimer James H Leebens-Mack Douglas E Soltis Pamela S Soltis Victor A Albert |
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Affiliation: | (1) Natural History Museum, University of Oslo, PO Box 1172, Blindern, 0318, Oslo, Norway;(2) Department of Botany, University of Florida, Gainesville, FL 32611, USA;(3) Department of Biology and Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA;(4) Florida Museum of Natural History, University of Florida, Gainesville, FL 32611, USA |
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Abstract: | Background The endemic Hawaiian mints represent a major island radiation that likely originated from hybridization between two North American polyploid lineages. In contrast with the extensive morphological and ecological diversity among taxa, ribosomal DNA sequence variation has been found to be remarkably low. In the past few years, expressed sequence tag (EST) projects on plant species have generated a vast amount of publicly available sequence data that can be mined for simple sequence repeats (SSRs). However, these EST projects have largely focused on crop or otherwise economically important plants, and so far only few studies have been published on the use of intragenic SSRs in natural plant populations. We constructed an EST library from developing fleshy nutlets of Stenogyne rugosa principally to identify genetic markers for the Hawaiian endemic mints. |
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