PARTIAL CHARACTERIZATION OF SOLUBLE ACETYLCHOLINESTERASE ISOENZYMES OF THE RAT BRAIN |
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Authors: | J. Bajgar, V. i kovský |
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Affiliation: | PurkyněMedical Research Institute, Hradec Královéand Institute of Toxicology and Forensic Chemistry, Charles University, Prague, Czechoslovakia |
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Abstract: | (1) The 105,000 g supernatant fluid obtained from rat brain was separated by agar-gel electrophoresis. (2) Three isoenzymes, capable of hydrolysing acetylthiocholine, one of them also hydrolysing butyrylthiocholine, were detected. (3) The pH optima and Km for hydrolysis of acetyl- and butyrylthiocholine by the supernatant fluid were determined. (4) After extraction of acetylcholinesterase isoenzymes from the gel, individual isoenzymes were characterized by pH optima and Km values. (5) Two of the enzymes were characterized as acetylcholinesterase (EC 3.1.1.7) and one as butyrylcholinesterase (EC 3.1.1.8). |
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