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Enzymatic and non-enzymatic assay of superoxide dismutase.
Authors:R Fried
Affiliation:Centre de Neurochimie du C.N.R.S., 11 rue Humann, 67085 Strasbourg Cedex, France
Abstract:Superoxide dismutase from breef brain and rat liver was assayed in an enzymatic system, using xanthine oxidase, and a non-enzymatic system, based on aerobic reduction of nitro-blue tetrazolium in presence of phenazine methosulphate. The non-enzymatic assay is rapid and simple and permits simulatneous analysis of many samples. Similar results are found by the two methods of assay of superoxide dismutase.
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