NaCl stress causes changes in photosynthetic pigments,proteins, and other metabolic components in the leaves of a true mangrove,<Emphasis Type="Italic">Bruguiera parviflora</Emphasis>, in hydroponic cultures |
| |
Authors: | Asish?Parida Email author" target="_blank">Anath?Bandhu?DasEmail author Premananda?Das |
| |
Institution: | (1) National Institute for Plant Biodiversity Conservation and Research and Regional Plant Resource Centre, Bhubaneswar 751015, Orissa, India |
| |
Abstract: | We studied salt stress-induced biochemical changes in young, hydroponically grown plants of mangrove,Bruguiera parviflora (Rhizophoraceae). Our focus was on the effect of NaCI (applied at 100, 200, 400, or 500 mM) on leaf pigments, total soluble
proteins, total free amino acids, carbohydrates, polyphenols, and proline. The total Chi content increased for 14 d after
treatment with 100 mM NaCI, then gradually stabilized. At 400 mM, the total Chi content slowly decreased over the 45-d test
period. However, the Chia:b ratio remained unchanged in isolated chloroplasts and in leaf tissue. Percent changes in the carotenoids content followed
the same trend as for Chi, except for a 1.5-fold decrease during the 400-mM NaCI treatment, compared with the control. The
total sugar content increased by 2.5-fold by Day 45 after treatment with 400 mM NaCI, whereas the starch content measured
in the same treatment decreased by 40 to 45%. Leaf protein content decreased as salinity increased, which suggests either
a possible disruption in the protein synthesis mechanism or, more likely, an increase in proteolytic activity. The total amino-acid
pool increased steadily, by four-fold, in the 45-d, 400-mM treatment Both proline and polyphenols accumulated with increasing
levels of salinity, which confirms the role of proline as a stress-induced protective metabolite in the adaptive process of
this species. Our results showed that a true mangrove such as 8.parviflora can easily be sustained and propagated under low-salinity conditions. At high levels of salinity (~400 mM, beyond which they
could not survive), the plants became adapted to salt stress after two to three weeks. During this adaptive period, changes
in pigment and protein levels also occurred. The accumulation of proline and polyphenols played a key role in the plant’s
stressinduced adjustment to NaCI under hydroponic culture conditions. |
| |
Keywords: | Bruguiera parviflora hydroponic culture mangrove proline accumulation salt-stress |
本文献已被 SpringerLink 等数据库收录! |
|