Determination of 3-methoxy-4-hydroxyphenylglycol in urine by high-performance liquid chromatography with amperometric detection |
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| Authors: | Rafael Alonso Candace J. Gibson James McGill |
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| Affiliation: | 1. Laboratory of Neuroendocrine Regulation Department of Nutrition and Food Science Massachusetts Institute of Technology Cambridge, Massachusetts 02139, USA;1. Departamento de Fisiologia y Bioquimica Facultad de Medicina Universidad de La Laguna La Cuesta, Tenerife (Canary Islands), Spain |
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| Abstract: | A reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of 3-methoxy-4-hydroxyphenylglycol (MHPG) in urine. After incubation with glusulase, free MHPG is extracted into ethyl acetate and further isolated by a combination of thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). The addition of amperometric detection provides increased sensitivity to a highly specific assay. |
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| Keywords: | Author to whom reprint requests should be sent. |
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