Graphical rules for non-steady state enzyme kinetic

The exiting graphical methods in enzyme kinetics can be used only within the scope of steady state reactions. In this paper, two graphical rules are presented to deal with the non-steady state enzyme catalysed reaction systems. According to Rule 1 we can immediately write out the phase concentration of enzyme species. The calculation work such as setting up differential equations, making Laplace transformation, expanding determinants, which are both tedious and liable to error, are completely saved. By means of Rule 2 the secular equations for the consecutive first-order reactions can be written out directly without need of setting up differential equations, expanding determinants, etc., that would otherwise be laborious and prone to errors. In addition, two check formulae are also presented for these two graphical methods, respectively. They are useful in order for avoiding the omission of terms during calculations, especially, for complicated mechanisms.