EFFECT OF SALTS ON THE PHYSICAL AND KINETIC PROPERTIES OF HUMAN PLACENTAL CHOLINE ACETYLTRANSFERASE |
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Authors: | Louis B Hersh Martha Peet |
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Institution: | Department of Biochemistry, The University of Texas Health Science Center at Dallas, Dallas, TX 75235, U.S.A. |
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Abstract: | Abstract— The effects of salt on the properties of human placental choline acetyltransferase have been examined. Increases in enzyme activity, thermal denaturation and susceptibility to proteolysis can be related to increases in ionic strength, rather than to specific salt effects. Increased ionic strength increases the maximal velocity (Km) of the reaction, with no change in the kinetic parameter Vmax/Km (choline). The pH-Km profile, measured over the range of 6.5–8.0, indicates the requirement of a dissociated acidic residue whose pKa is below 7.5 at high ionic strength, and a protonated residue whose pKa is above 7.5 at low ionic strength. It is proposed that the conformation of the enzyme is different at high ionic strength and at low ionic strength, and that these different conformational states of the enzyme result in different rate-determining steps of the reaction. |
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