Improved (R)-phenylacetylcarbinol production with Candida utilis pyruvate decarboxylase at decreased organic to aqueous phase volume ratios |
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Authors: | Cindy Gunawan Michael Breuer Bernhard Hauer Peter L. Rogers Bettina Rosche |
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Affiliation: | (1) School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia;(2) BASF-AG, Fine Chemicals and Biocatalysis Research, 67056 Ludwigshafen, Germany |
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Abstract: | The effect of decreasing the organic (octanol) to aqueous phase volume ratio was evaluated in a two-phase enzymatic process for (R)-phenylacetylcarbinol (PAC) production. Decreasing the ratio from 1:1 to 0.43:1 at 4°C increased PAC in the organic phase from 112 g/l to 183 g/l with a 10% improvement in overall productivity. Interestingly, the rate of enzyme (pyruvate decarboxylase) activity loss was unaffected by the reduced phase ratio over the reaction period (48 h). At 20°C and 0.43:1 phase ratio the organic phase PAC concentration increased to 212 g/l and the overall productivity increased by 30% although the PAC yield (based on pyruvate) declined by about 10% due to greater byproduct acetoin formation at the higher temperature. Product recovery in such a system is facilitated both by the higher PAC concentration and the reduced organic phase volume. |
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Keywords: | Biotransformation Decreased organic:aqueous phase ratio Pyruvate decarboxylase (R)-Phenylacetylcarbinol Two-phase process |
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