A rapid radioenzymatic assay for dopamine and N-acetyldopamine. |
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| Authors: | M W McCaman R E McCaman J Stetzler |
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| Affiliation: | Laboratory of Molecular Neurobiology, Division of Neurosciences, City of Hope National Medical Center, 1500 East Duarte Road, Duarte, California 91010 USA |
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| Abstract: | Dopamine (DA) was measured in various tissue extracts as [3H]methoxy-N-acetyldopamine after incubation with two partially purified enzymes, catechol-O-methyl transferase (EC 2.1.1.1) and N-acetyltransferase (EC 2.3.1.5), in the presence of [3H]adenosylmethionine and acetyl-CoA. This product can be separated quantitatively from labeled products of norepinephrine and epinephrine by solvent extraction. N-Acetyl-DA can be assayed by omitting the acetylating system from the incubation mixture. The procedure is rapid, convenient for processing large numbers of samples, and has a sensitivity of approximately 0.1 pmol. It has been used to measure DA in ganglia and in individual neurons from gastropod mollusks. |
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| Keywords: | To whom correspondence should be addressed. |
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