13C-NMR study of lactate metabolism in Propionibacterium freudenreichii subsp. shermanii |
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| Authors: | D. B. Rolin F. Girard J. D. de Certaines P. Boyaval |
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| Affiliation: | (1) Université de Rennes I, Laboratoire de Biologie végétale, av. du Général Leclerc, 35042 Rennes Cedex, France;(2) Institut Technique du Gruyère, 73 rue de St Brieuc, BP 6224, 35062 Rennes Cedex, France;(3) Université de Rennes I, Laboratoire de RMN en Biologie et Médecine, Faculté de Médecine, av. Prof. L. Bernard, 35043 Rennes Cedex, France;(4) Laboratoire de Recherche en Technologie Laitière, Institut National de la Recherche Agronomique, 65 rue de Saint-Brieuc, 35042 Rennes Cedex, France |
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| Abstract: | The adaptation to osmotic stress in Propionibacterium freudenreichii subsp. shermanii was investigated by using natural-abundance 13C nuclear magnetic resonance spectroscopy. Cells incubated either in a standard laboratory medium or in a medium designed to simulate the physicochemical conditions of Swiss-type cheese were found to accumulate different levels of osmotic-stress-protectant molecules. Proline, betaine, trehalose and glutamate were found simultaneously. Moreover, two types of polysaccharides were in evidence in this strain. Lactate catabolism was not mainly directed towards cell growth requirements and organic acid production but also towards biosynthesis of osmolytes requested for adaptation in a cheese environment. The possible involvement of such type of metabolite accumulation in the main cheese-ripening bacteria in Swiss-type cheeses is discussed. |
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