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Continuous production of lignin-degrading enzymes by Bjerkandera adusta immobilized on polyurethane foam
Authors:Godliving Mtui  Yoshitoshi Nakamura
Affiliation:(1) Applied Microbiology Unit, University of Dar es Salaam, P.O. Box 35060, Dar es Salaam, Tanzania;(2) Department of Chemistry and Chemical Engineering, Faculty of Engineering, Kanazawa University, 2-40-20 Kodatsuno, Kanazawa, 920-8667, Japan
Abstract:Continuous production of lignin-degrading enzymes by Bjerkandera adusta immobilized on polyurethane foam gave maximum activities of 220 U lignin peroxidase ml–1, 150 U manganese peroxidase ml–1, 50 U laccase ml–1 and 6.2 U protease ml–1 at the retention time of 24 h for 60 days. Protease secretion destabilized the produced lignin peroxidase, manganese peroxidase and laccase.
Keywords:Bjerkandera adusta  continuous production  immobilized culture  lignin-degrading enzymes  polyurethane foam
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