Metachromasy of peripheral nerve collagen on polyacrylamide gels stained with Coomassie brilliant blue R-250. |
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Authors: | S Micko W W Schlaepfer |
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Institution: | Department of Pathology, Washington University School of Medicine, 660 South Euclid, St. Louis, Missouri 63110 USA |
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Abstract: | Endoneurial collagen stains metachromatically with Coomassie brilliant blue R-250 (C.I. 42660) when peripheral nerve proteins are solubilized with urea and SDS and then subjected to SDS-polyacrylamide gel electrophoresis. The metachromasy is reproducible under different fixing and staining conditions, but was exhibited only by Coomassie blue R-250 of the four triphenylmethane dyes tested. A method is presented for measurement of the degree of metachromasy on SDS gels and the detection of collagen in homogenates of whole tissue. |
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