| 蛋白质定向进化的研究进展及其应用前景 |
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| 引用本文： | 许钦坤,王红宁,赵翠燕,李洪淼. 蛋白质定向进化的研究进展及其应用前景[J]. 生物技术通讯, 2005, 16(2): 191-193 |
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| 作者姓名： | 许钦坤 王红宁 赵翠燕 李洪淼 |
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| 作者单位： | 1. 四川农业大学,动物科技学院,四川,雅安,625014
2. 韶关学院,英东生物工程学院,广东,韶关,512005 |
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| 基金项目： | 国家“十五”重点科技攻关子课题(2002BA514A-12) |
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| 摘 要： | 定向进化是改造蛋白质分子的有效新策略.它不需要了解蛋白质的空间结构,主要通过在实验室里模拟自然进化过程,采用错误倾向PCR等方法对编码蛋白质的基因进行随机突变,经DNA改组、交错延伸等技术进行体外重组,设计高通量筛选方法来选出需要的突变体.本综述了定向进化技术的发展及应用.
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| 关 键 词： | 定向进化 错误倾向PCR DNA改组 蛋白质 基因突变 |
| 文章编号： | 1009-0002(2005)02-0191-03 |
| 修稿时间： | 2004-09-10 |
Advances in Research and Application on Directed Evolution of Proteins |
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| XU Qin-kun,WANG Hong-ning,ZHAO Cui-yan,LI Hong-miao. Advances in Research and Application on Directed Evolution of Proteins[J]. Letters in Biotechnology, 2005, 16(2): 191-193 |
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| Authors: | XU Qin-kun WANG Hong-ning ZHAO Cui-yan LI Hong-miao |
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| Affiliation: | XU Qin-kun1,WANG Hong-ning1,ZHAO Cui-yan2,LI Hong-miao11. College of Animal Technology and Science,Sichuan Agriculture University,Ya'an 625014,2. College of Yingdong Bioengineering,Shaoguan College,Shaoguan 512005,China |
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| Abstract: | Directed evolution is a new powerful strategy for engineering proteins. It is independent of any knowledge concerning the structure or the mechanism of the protein being evolved, it mainly mimics the process of natural evolution in the laboratory to generate random mutations in the genes coding for useful proteins by the techniques of error prone PCR and DNA shuffling, then in vitro recombines positive mutations through such methods as high-fidelity DNA shuffling and staggered extension process. Finally, the desired mutants are selected out with high-throughput screening. Its development and applications were reviewed in this paper. |
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| Keywords: | directed evolution error prone PCR DNA shuffling |
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