Application of terminal restriction fragment length polymorphism (T-RFLP) analysis to monitor effect of biocontrol agents on rhizosphere microbial community of hot pepper (<Emphasis Type="Italic">Capsicum annuum</Emphasis> L.) |
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Authors: | Young Tae Kim Myoungho Cho Je Yong Jeong Hyang Burm Lee Seung Bum Kim |
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Institution: | (1) Department of Biological Sciences, University of Idaho, Life Science South, Room 463, Moscow, ID 83844, USA;(2) Department of Mathematics, University of Idaho, Moscow, ID 83844, USA;(3) Department of Statistics, University of Idaho, Moscow, ID 83844, USA;(4) Department of Physics, University of Idaho, Moscow, ID 83844, USA;(5) Initiative for Bioinformatics and Evolutionary Studies, University of Idaho, Moscow, ID 83844, USA; |
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Abstract: | Microbial communities in hot pepper (Capsicum annuum L.) cultivation fields under different cultivation methods were investigated by terminal restriction fragment length polymorphism
(T-RFLP) analysis. Rhizosphere soil and leaf samples were collected from control, conventional and nature-friendly cultivation
fields between May and July, 2009. Two Bacillus subtilis strains were applied to nature-friendly cultivation fields as biocontrol agents during the sampling period. Relative abundances
of bacteria and plant pathogenic fungi related T-RFs were also measured to monitor the effect of biocontrol agents on potential
plant pathogenic fungi. In the principal component analysis (PCA) based on T-RFLP profiles, the microbial communities from
rhizosphere soil samples in July, including bacteria and fungi, showed distinct difference between nature-friendly cultivation
fields and other cultivation fields. However, there was no correlation between cultivation methods and leaf microbial communities
at any sampling period. Changes in the abundance of bacteria related T-RF in the rhizosphere of nature-friendly cultivation
fields were observed clearly two months after application of biocontrol agent, while the abundance of plant pathogenic fungi
related T-RFs significantly decreased. |
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