NPGB-induced inhibition of superoxide anion production by normal Lewis rat macrophages |
| |
Authors: | Arduino Arduini Giovanna Mancinelli Maurizio Belfiglio Jerome DeJulia Valter Damonti Stefano Storto Giorgio Federici |
| |
Affiliation: | (1) Istituto di Scienze Biochimiche, Facoltá di Medicina, Universitá degli Studi G. D'Annunzio , 66100 Chieti, Italy;(2) Dipartimento di Biologia, Universitá di Tor Vergata , Via Orazio Raimondo, 00173 Roma, Italy |
| |
Abstract: | The treatment of Lewis rat peritoneal macrophages with p1-nitrophenyl p-guanidinobenzoate (NPGB) inhibited the superoxide anion production stimulated with phorbol myristate acetate (PMA). The addition of NPGB at the time of maximum superoxide generation was still able to block the superoxide release. It appears from these findings that NPGB may block either the activation process of the membrane bound NAD(P)H oxidase or directly on the active enzyme. Other protease inhibitors such as, epsilon-amino caproic acid (EACA), pepstatin, trans aminomethyl cyclohexane carboxylic acid (AMCA), aprotinin, and leupeptin did not inhibit the superoxide release. The superoxide anion release by the xanthine-xanthine oxidase system was not inhibited by NPGB. This finding indicates that NPGB does not itself react with superoxide. It has been also demonstrated that NPGB is a good reactant toward sulfhydryl group. The relevance of these finding to experimental allergic encephalomyelitis (EAE) is discussed. |
| |
Keywords: | Lewis rat macrophages superoxide anion NADPH-oxidase p-nitrophenyl p /content/u637638u72243647/xxlarge8242.gif" alt=" prime" align=" BASELINE" BORDER=" 0" >-guanidinobenzoate protease inhibitors |
本文献已被 SpringerLink 等数据库收录! |
|