| 早期先天梅毒几种实验室诊断方法的评价 |
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| 引用本文： | 袁小松,张一鸣,蒋丽霞.早期先天梅毒几种实验室诊断方法的评价[J].中国微生态学杂志,2010,22(2):169-170. |
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| 作者姓名： | 袁小松 张一鸣 蒋丽霞 |
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| 作者单位： | 常州市妇幼保健院,检验科,江苏,常州,213003 |
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| 摘 要： | 目的评价梅毒螺旋体蛋白印迹试验(TPPA-IgM-WB)和梅毒螺旋体19(s)-IgM酶联免疫吸附法TP-19(s)-IgM-ELISA]在先天梅毒早期诊断中的作用。方法对45例梅毒孕妇所产46例(1例双胞胎)新生儿运用血清IgM-WB试验、梅毒螺旋体19(s)-IgM酶联免疫吸附法TP-19(s)-IgM-ELISA]和常规血清学方法(TPPA、RPR、FTA-ABS-IgM)检测,评价上述试验诊断方法在先天梅毒早期诊断中的作用。结果45例梅毒孕妇所生的新生儿中,按常规综合诊断方法21例确诊为先天梅毒,新生儿血清IgM蛋白印迹试验23例阳性,梅毒螺旋体19(s)-IgM酶联免疫吸附法24例阳性(21例常规方法诊断为先天梅毒)。30例作为对照的非梅毒孕妇及新生儿各项检查均为阴性。结论血清IgM蛋白印迹试验和梅毒螺旋体19(s)-IgM酶联免疫吸附法TP-19(s)-IgM-ELISA]诊断先天梅毒具有较高的特异性和敏感性,结果显示可能高于现行的常规综合诊断方法的诊断价值。
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| 关 键 词： | 先天梅毒 梅毒螺旋体 诊断 血清IgM蛋白印迹试验 梅毒螺旋体19（s）OIgM酶联免疫吸附法 |
Evalution of detection of serum IgM Treponema pallidum by several methods in the diagnosis of early congenital syphilis |
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| YUAN Xiao-song,ZHANG Yi-ming,JIANG Li-xia.Evalution of detection of serum IgM Treponema pallidum by several methods in the diagnosis of early congenital syphilis[J].Chinese Journal of Microecology,2010,22(2):169-170. |
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| Authors: | YUAN Xiao-song ZHANG Yi-ming JIANG Li-xia |
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| Institution: | YUAN Xiao-song,ZHANG Yi-ming,JIANG Li-xia (Laboratory Department of Women , Children\'s Healthcare Center of Changzhou,Changzhou 213003,China) |
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| Abstract: | Objective To evaluate the effects of TPPA-IgM-WB and TP-19(s)-IgM-ELISA in the detection of Treponema pallidum IgM for diagnosis of congenital syphilis.Method Methods of TPPA-IgM-WB,TP-19(s)-IgM-ELISA,FTA-ABS-IgM,TRUST and TPPA were used simultaneously to test sera of 46 neonates born to 45 women with syphilis.Result Positive TP-19(s)-IgM-ELISA(including 21 congenital syphilis) were observed in 24 out of the 46 serum specimens from syphilitic women;23 positive serum IgM were detected by Western blot(21 cong... |
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| Keywords: | Congenital syphilis Treponema pallidum ingnosis TPPA-IgM-WB TP-19(s)-IgM-ELISA |
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