Study of the synthesis, antiproliferative properties, and interaction with DNA and polynucleotides of cisplatin-like Pt(II) complexes containing carcinogenic polyaromatic amines |
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Authors: | Elisabetta Gabano Sofia Gama Filipa Mendes Marzia B. Gariboldi Elena Monti Sophie Bombard Sabrina Bianco Mauro Ravera |
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Affiliation: | 1. Dipartimento di Scienze e Innovazione Tecnologica (DiSIT), Università del Piemonte Orientale “Amedeo Avogadro”, Viale Michel 11, 15121, Alessandria, Italy 2. Unidade de Ciências Químicas e Radiofarmacêuticas, Instituto Superior Técnico-Campus Tecnológico e Nuclear, Estrada Nacional 10 (km 139,7), 2695-066, Bobadela LRS, Portugal 3. Dipartimento di Scienze Teoriche e Applicate (DiSTA), Università dell‘Insubria, Via H. Dunant 3, 21100, Varese, Italy 4. Université Paris Descartes, INSERM UMR-S-1007, 45 rue des Saints-Pères, 75006, Paris, France
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Abstract: | The chemical and biological features of two newly synthesized [PtCl2(L)(2-aminonaphthalene)] complexes (L is NH3 or 2-aminonaphthalene) were compared with those of two already reported enantiomeric complexes of formula [PtCl2(DABN)] [DABN is (R)-1,1′-binaphthyl-2,2′-diamine or (S)-1,1′-binaphthyl-2,2′-diamine]. Solution behavior, lipophilicity, cytotoxicity with regard to one colorectal (HCT116) and two ovarian (A2780 and A2780Cp8) human carcinoma cell lines, and in vitro DNA- and G-quadruplex-binding properties were evaluated. In particular, the cytotoxicity of [PtCl2(NH3)(2-aminonaphthalene)] was better than that of cisplatin for all cell lines, and rather resembled that of oxaliplatin. The solution behavior of the whole series of complexes and the absence of an evident relationship between lipophilicity and cytotoxicity seem to suggest that all these experimental parameters are probably smoothed out during the 3-day cytotoxicity experiments and do not strongly affect the half-maximal inhibitory concentrations. The results of electrophoretic studies indicate that different kinds of interaction with DNA can be involved in the mode of action of these complexes, with intercalation in double-stranded DNA and stacking on G-quadruplex DNA being strongly implicated in particular for [PtCl2(NH3)(2-aminonaphthalene)]. |
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