Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays |
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Authors: | Fierer Noah Jackson Jason A Vilgalys Rytas Jackson Robert B |
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Affiliation: | Department of Biology, Duke University, Durham, North Carolina 27708, USA. fierer@lifesci.ucsb.edu |
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Abstract: | Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community structure. |
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