| 细胞色素P450 2D6酶缺陷等位基因的分析 |
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| 引用本文： | 陈枢青,孙红颖,赵鲁杭,P.J.WEDLUND. 细胞色素P450 2D6酶缺陷等位基因的分析[J]. 生物化学与生物物理进展, 1999, 26(1): 90-92 |
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| 作者姓名： | 陈枢青 孙红颖 赵鲁杭 P.J.WEDLUND |
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| 作者单位： | 1. 浙江医科大学生物化学教研室,杭州,310006
2. College of Pharmacy, University of Kentucky, Lexington KY 40536 USA |
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| 基金项目： | 浙江省卫生厅科研基金资助项目(1996年). |
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| 摘 要： | 细胞色素P450 2D6(CYP2D6)第1 795位胸腺嘧啶核苷缺失造成CYP2D6酶活性缺陷，该等位基因被称为CYP2D6T.对该等位基因的测定有助于准确预测CYP2D6表现型.利用等位基因特异扩增法的基本原理，建立了测定CYP2D6T的方法.经396例测定，证明比利用PCR扩增后再酶切的方法更为快捷、更少污染，为该项测定应用于临床奠定基础.
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| 关 键 词： | 细胞色素P450 2D6(CYP2D6)，基因分型，CYP2D6T，等位基因特异扩增法 |
| 收稿时间： | 1997-11-10 |
| 修稿时间： | : 1997-11- |
Analysis of Cytochrome P450 2D6 Enzyme Deficient Allele CYP2D6T by Allele-Specific Amplification |
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| CHEN Shu-Qing,SUN Hong-Ying,ZHAO Lu-Hang and P.J.WEDLUND. Analysis of Cytochrome P450 2D6 Enzyme Deficient Allele CYP2D6T by Allele-Specific Amplification[J]. Progress In Biochemistry and Biophysics, 1999, 26(1): 90-92 |
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| Authors: | CHEN Shu-Qing SUN Hong-Ying ZHAO Lu-Hang P.J.WEDLUND |
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| Abstract: | An allele-specific amplification based PCR method was built up for the detection of CYP2D6T allele which is a 1 795 T deletion on CYP2D6 gene and results in a function deficient enzyme. This method was employed to genotype 396 subjects and proved to be simple, quick and less contamination. It has made a basis for this detection a clinical tool. |
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| Keywords: | cytochrome P450 2D6 genotyping CYP2D6T allele-specific amplification |
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