AtSTP3启动子控制的外源基因在转基因水稻中的特异表达研究

利用PCR技术从哥伦比亚型拟南芥基因组DNA中分离了AtSTP3绿色组织特异表达的启动子,序列分析表明,扩增片段(1774bp)与已报道序列的相应区域同源性达99.9%。将其与GUS报告基因融合在一起,构建了植物表达载体,并由农杆菌介导法导入水稻品种‘中花11’中。对转基因水稻植株中的GUS活性进行定性与定量测定结果表明,AtSTP3启动子可驱动GUS报告基因在转基因水稻植株叶片中特异性表达,而在根和种子等器官中不表达或表达活性极弱,AtSTP3启动子表现出明显的组织特异性。

Specific Expression of Exogenous Genes Regulated by AtSTP3 Promoter of Arabidopsis thaliana

AtSTP3 promoter,which expressed specifically in green tissues,was isolated from Arabidopsis thaliana of Colombia type By PCR and its sequencing indicated that the amplified band (1 744 bp) was 99.9% homologous to the reported ones at the correspondent sequence regions.It was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into Zhonghua 11,a rice variety,by Agrobacterium meditation.The determinations of the GUS activities in the transgenic rice plants indicated AtSTP3 promoter could drive the GUS reporter gene to express in the leaves of the transgenic rice plants while exerting no or very weak influence on the expression of the GUS reporter gene,and so AtSTP3 promoter was tissue specific in its expression.